Identification and immunochemical characterization of a germ tube specific antigen ofCandida albicans
نویسندگان
چکیده
منابع مشابه
New monoclonal antibody specific for Candida albicans germ tube.
Hydrophobic components of the germ tube of the dimorphic pathogenic fungus Candida albicans were used as immunogens to prepare monoclonal antibodies (MAbs). Among the resulting MAbs, one (MAb 16B1-F10) was shown by indirect immunofluorescence to be specific to the surface of the mycelium phase of the C. albicans and C. stellatoidea species. No labeling of any other genera and Candida species te...
متن کاملIdentification, cloning, and initial characterization of a novel mouse testicular germ cell-specific antigen.
A monoclonal antibody, designated TES101, was raised by immunizing BALB/c mice with an allogenic mouse testicular homogenate followed by immunohistochemical selection as the initial screening method. By searching the expressed sequence tag (EST) database with the N-terminal amino acid sequence of TES101 reactive protein, we found that the predicted amino acid sequence encoded by a mouse testicu...
متن کاملIdentification and purification of a specific and immunogenic antigen of the laminated layer of the hydatid cyst and production of an antigen-specific monoclonal antibody.
Cystic echinococcosis (CE) is an infection caused by the larval stage of Echinococcus granulosus. This is widely distributed through Iran, where a variety of animals act as intermediate host. The immunogenic antigens (Ag) of different compartments of the hydatid cyst have been already determined. One of these compartments is the laminated layer (LL). We have extracted a protein with the MW of 2...
متن کاملIdentification of the dialysable serum inducer of germ-tube formation in Candida albicans.
Yeast cells of Candida albicans are induced by serum at 37 degrees C to produce germ tubes, the first step in a transition from yeast to hyphal growth. Previously, it has been shown that the active component is not serum albumin but is present in the dialysable fraction of serum. In this study, serum induction of germ-tube formation is shown to occur even in the presence of added exogenous nitr...
متن کاملSerum tube identification of Candida albicans.
The production of filaments (germ tubes) by cells of Candida albicans in serum tubes permits presumptive identification to be made within two to three hours. The proportion of yeast-like cells forming filaments is progressively decreased with increasing cell concentration. The test is effective over a comparatively wide range of temperatures and using different types and concentrations of sera.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEMS Immunology & Medical Microbiology
سال: 1993
ISSN: 0928-8244,1574-695X
DOI: 10.1111/j.1574-695x.1993.tb00397.x